EpiMelt MGMT test assay

Homo sapiens O-6-methylguanine-DNA methyltransferase (MGMT), NM_002412

DNA Methylation Assay Kit for MGMT based on MS-HRM. Includes controls.

Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of MGMT templates supplied with the MethylDetect MGMT kit.

Results obtained following the MethylDetect MGMT kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of MGMT templates supplied with the MethylDetect MGMT kit.

Results obtained following the MethylDetect MGMT kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.