EpiMelt CDKN2B test assay

Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), NM_004936

DNA Methylation Assay Kit for CDKN2B, based on MS-HRM. Includes controls,

 

Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of CDKN2B templates supplied with the MethylDetect CDKN2B kit.

Results obtained following the MethylDetect CDKN2B kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of CDKN2B templates supplied with the MethylDetect CDKN2B kit.

Results obtained following the MethylDetect CDKN2B kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.