EpiMelt CDKN2B test assay

Homo sapiens cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) (CDKN2B), NM_004936

DNA Methylation Assay Kit for CDKN2B, based on MS-HRM. Includes controls,

 

EpiMelt test assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 5 sets of ready to use controls
EpiMelt test assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 1 set of ready to use controls
EpiMelt test assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 1 set of ready to use controls
EpiMelt Real-Time PCR Master Mix 200 reactions, E
EpiMelt Bisulfite Modification Standard Kit 200 reactions, F

Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of CDKN2B templates supplied with the MethylDetect CDKN2B kit.

Results obtained following the MethylDetect CDKN2B kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of CDKN2B templates supplied with the MethylDetect CDKN2B kit.

Results obtained following the MethylDetect CDKN2B kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.