EpiMelt Real-Time PCR Master Mix
Optimized for EpiMelt Methylation Detection Assays to ensure maximum sensitivity and reliable qPCR performance.
Normalized amplification curves and relative signal difference (-d/dT) plot illustrating positive control (red), assay calibration control (blue), and negative control (orange) from an EpiMelt Methylation Detection Assay after PCR using EpiMelt Real-Time PCR Master Mix. The EpiMelt Real-Time PCR Master Mix is a high-specificity, ready-to-use reagent for real-time Hot-Start PCR for melting analysis. Aside from DNA template and primers, the EpiMelt Master Mix contains all components required for real-time PCR, including the EpiMelt Hot Start DNA Polymerase.
The Hot Start DNA Polymerase is a chemically blocked DNA polymerase that is inactive at room temperature, preventing unspecific extension during setup. This master mix premix formulation saves time and reduces contamination.
