MethylDetect introduces:

EpiMelt DNA Methylation Testing Kits

DNA Methylation is one of the most widely studied epigenetic mechanisms of gene expression regulation. Aberrant methylation of increasing number of genes is being shown to contribute to the majority of diseases such as cancer, cardiovascular disorders, aging, obesity, allergy, diabetes, depression, Alzheimer’s, Parkinson’s or schizophrenia.

Solid research evidence already shows that DNA methylation-based biomarkers can be utilized at all stages of clinical disease management including:

• Disease risk assessment
• Early detection
• Personalization of treatment
• Monitoring of chronic disorders and recurrence

The technology is already in use for detection of DNA methylation biomarkers in fields like forensics, and for investigating challenging DNA materiel sources as liquid biopsies and FFPE tissues.

MethylDetect offers ready-to-use kits for detection of aberrant gene methylation. The kits are based on the MS-HRM (Methylation Sensitive High Resolution Melting) technology and can be used with standard laboratory equipment. Additionally, our new controls system shipped with each kit, provides state-of-the-art experimental reliability.

More about epigenetics, methylation and our technology in: “Resources“

DNA Methylation detection using the EpiMelt kit

1. ADD:

The MethylDetect kit components and the bisulfite modified biological samples to the PCR reagents

2. AMPLIFY:

Use a standard laboratory Real Time-PCR system to PCR amplify the target.

3. DETECT:

Assess the methylation status of the sequence of intereest with a High-Resolution Melting module.