Resources

Resources

Resources for MS-HRM

 

Here you will find some of our resources concerning MS-HRM:

Application Notes

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Application Note: Bisulfite Modification Standard Kit

High bisulfite conversion efficiency using EpiMelt Bisulfite Modification Standard Kit on genomic DNA…

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Application Note: EpiMelt Real-Time PCR Master Mix

High-sensitivity EpiMelt DNA methylation analysis using EpiMelt Real-Time PCR Master Mix…

Protocols

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Protocol: Bisulfite Modification Standard Kit

Complete kit for efficient conversion and preparation of converted DNA for methylation research…

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Protocol: Assay Protocol

The performance of the EpiMelt Methylation Detection Assay has been tested on the…

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Protocol: EpiMelt Real-Time PCR Master Mix

High specificity ready-to-use for real-time Hot Start PCR with EvaGreen®. Optimized for epigenetic…

Other

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What Is Epigenetics?

Epigenetics is a study of the heritable gene expression changes that do not involve alternations in DNA sequence…

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What Is Bisulfite Modification?

During DNA replication (cell division) methyl groups (DNA methylation) are resynthesized on the newly replicated…

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What Is DNA Methylation?

Enzymatic addition of methyl groups (CH3-) to the DNA molecule is referred to as DNA methylation…

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What Is DNA Melting?

Dissociation of the double stranded DNA helix into single coils is referred to as DNA melting…

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How Does MS-HRM Work?

The number of cytosines (C) in a PCR product, amplified from a sodium bisulfite modified template, depends on the…

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PCR Bias In Methylation Experiments

The methylated and non-methylated locus after bisulfite modification differs in number of cytosines…

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Controls In Methylation Detection Experiments

Following good laboratory practice any experiment needs to be properly controlled…

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MS-HRM For Methylation Detection In FFPE Material

Formalin-Fixation followed by paraffin-embedment (FFPE) is one of the most widely practiced methods for…

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MS-HRM For Methylation Detection In Liquid Biopsies

Tumor DNA is secreted into body fluids that come in direct contact with the tumor site…

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DNA Methylation In Cancer

Methylation of mammalien cell DNA is the most studied, well-established epigenetic mechanism…

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Methylation Diabetes

The epigenome is determined by DNA methylation and histone modifications, which together is…

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Methylation In Lung Cancer

Lung cancer is the leading cause of cancer related deaths world wide. Often patients with lung cancer…

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MGMT Gene

O6-methylguanine-DNA methyltransferase (MGMT) is a DNA repair enzyme, involved in cellular…

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Methylation Glioblastoma

Gliomas are the most common neoplasm of the central nervous system, they originate from glia cells…

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Methylation Liver Cancer

Liver cancer is one of the most lethal cancers worldwide. It has a highly aggressive disease course…

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MLH1 Gene

The MutL homolog 1 (MLH1) gene is a tumor suppressor gene involved in the DNA mismatch…

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BRCA2 Gene

The tumor suppressor gene BRCA2 encodes the BRCA2 protein, which is involved in…

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BRCA1 Gene

The breast cancer type 1 susceptibility gene (BRCA1) is a tumor suppressor gene involved in many…