Can be used with most methylation screening technologies including MS-HRM (Figure 1), MSP or bisulfite sequencing.

Can also be used to generate standard curves for technologies like q-MSP or MethyLight (Figure 2).

The control system includes:

  • non-methylated control – 0% methylation
  • fully methylated control – 100% methylation
  • any methylation level measurement points between 0% and 100% methylated allele in background of non-methylated allele

Figure 1:

Figure 2:

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