Can be used with most methylation screening technologies including MS-HRM (Figure 1), MSP or bisulfite sequencing.
Can also be used to generate standard curves for technologies like q-MSP or MethyLight (Figure 2).
The control system includes:
- non-methylated control – 0% methylation
- fully methylated control – 100% methylation
- any methylation level measurement points between 0% and 100% methylated allele in background of non-methylated allele