EpiMelt THBS1 test assay

Homo sapiens thrombospondin 1 (THBS1), NM_003246

DNA Methylation Assay Kit for THBS1 based on MS-HRM. Includes controls.

Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of THBS1 templates supplied with the MethylDetect THBS1 kit.

Results obtained following the MethylDetect THBS1 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of THBS1 templates supplied with the MethylDetect THBS1 kit.

Results obtained following the MethylDetect THBS1 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.