EpiMelt POU4F test assay

Homo sapiens POU class 4 homeobox 2 (POU4F2), NM_004575

DNA Methylation Assay Kit for TWIST, based on MS-HRM. Includes controls

 

EpiMelt test assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 5 sets of ready to use controls, A
EpiMelt test assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 1 set of ready to use controls, A
EpiMelt test assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 1 set of ready to use controls, A
EpiMelt test assay + EpiMelt Master Mix reactions – 200 – contains primermix for 200 MS-HRM PCRs, 1 set of ready to use controls and EpiMelt PCR master Mix, B
EpiMelt test assay + EpiMelt Master Mix reactions – 2500 - contains primermix for 2500 MS-HRM PCRs, 5 sets of ready to use controls and EpiMelt PCR Master Mix, B
EpiMelt test assay + EpiMelt Master Mix reactions – 500 – contains primermix for 500 MS-HRM PCRs, 1 set of ready to use controls and EpiMelt PCR master Mix, B
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Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of POU4F templates supplied with the MethylDetect POU4F kit.

Results obtained following the MethylDetect POU4F kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of POU4F templates supplied with the MethylDetect POU4F kit.

Results obtained following the MethylDetect POU4F kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

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