EpiMelt BRCA2 test assay

Homo sapiens breast cancer 2, early onset (BRCA2), NM_000059

DNA Methylation Assay Kit for BRCA2, based on MS-HRM. Includes controls

 

EpiMelt test assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 5 sets of ready to use controls
EpiMelt test assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 1 set of ready to use controls
EpiMelt test assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 1 set of ready to use controls
EpiMelt Real-Time PCR Master Mix 200 reactions, E
EpiMelt Bisulfite Modification Standard Kit 200 reactions, F

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.

Results obtained following the MethylDetect BRCA2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.

Results obtained following the MethylDetect BRCA2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.