Description
Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA1 templates supplied with the MethylDetect BRCA1 kit.
Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA1 templates supplied with the MethylDetect BRCA1 kit.
Results obtained following the MethylDetect BRCA1 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
