EpiMelt PTGS2 test assay

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Homo sapiens prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)(PTGS2), NM_000963

DNA Methylation Assay Kit for PTGS2 based on MS-HRM. Includes controls.

Product option: EpiMelt test assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 36 sets of ready to use controls, C: A
Product option: EpiMelt test assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 6 sets of ready to use controls, C: A
Product option: EpiMelt test assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 3 sets of ready to use controls, C: A
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 200 – contains primermix for 200 MS-HRM PCRs, 3 sets of ready to use controls and EpiMelt PCR master Mix, C: B
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 2500 - contains primermix for 2500 MS-HRM PCRs, 36 sets of ready to use controls and EpiMelt PCR Master Mix, C: B
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 500 – contains primermix for 500 MS-HRM PCRs, 6 sets of ready to use controls and EpiMelt PCR master Mix, C: B
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Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of PTGS2 templates supplied with the MethylDetect PTGS2 kit.

Results obtained following the MethylDetect PTGS2 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of PTGS2 templates supplied with the MethylDetect PTGS2kit.

Results obtained following the MethylDetect PTGS2 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

Additional information

C

A, B