EpiMelt CDH1 assay

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Homo sapiens cadherin 1, type 1, E-cadherin (epithelial) (CDH1), NM_004360

DNA Methylation Assay Kit for CDH1, based on MS-HRM. Includes controls

 

Product option: EpiMelt assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 36 sets of ready to use controls
Product option: EpiMelt assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 6 sets of ready to use controls
Product option: EpiMelt assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 3 sets of ready to use controls
Product option: EpiMelt Assay + EpiMelt Master Mix reactions – 200 – contains primermix for 200 MS-HRM PCRs, 3 sets of ready to use controls and EpiMelt PCR master Mix
Product option: EpiMelt Assay + EpiMelt Master Mix reactions – 2500 - contains primermix for 2500 MS-HRM PCRs, 36 sets of ready to use controls and EpiMelt PCR Master Mix
Product option: EpiMelt Assay + EpiMelt Master Mix reactions – 500 – contains primermix for 500 MS-HRM PCRs, 6 sets of ready to use controls and EpiMelt PCR master Mix
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Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of CDH1 templates supplied with the MethylDetect CDH1 kit.

Results obtained following the MethylDetect CDH1 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of CDH1 templates supplied with the MethylDetect CDH1 kit.

Results obtained following the MethylDetect CDH1 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.