EpiMelt BRCA2 test assay

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Homo sapiens breast cancer 2, early onset (BRCA2), NM_000059

DNA Methylation Assay Kit for BRCA2, based on MS-HRM. Includes controls

 

Product option: EpiMelt test assay 2500 reactions – containing primermix for 2500 MS-HRM PCRs and 36 sets of ready to use controls, C: A
Product option: EpiMelt test assay 500 reactions - containing primermix for 500 MS-HRM PCRs and 6 sets of ready to use controls, C: A
Product option: EpiMelt test assay 200 reactions – containing primermix for 200 MS-HRM PCRs and 3 sets of ready to use controls, C: A
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 200 – contains primermix for 200 MS-HRM PCRs, 3 sets of ready to use controls and EpiMelt PCR master Mix, C: B
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 2500 - contains primermix for 2500 MS-HRM PCRs, 36 sets of ready to use controls and EpiMelt PCR Master Mix, C: B
Product option: EpiMelt test assay + EpiMelt Master Mix reactions – 500 – contains primermix for 500 MS-HRM PCRs, 6 sets of ready to use controls and EpiMelt PCR master Mix, C: B
Clear

Description

Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.

Results obtained following the MethylDetect BRCA2 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

 

Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.

Results obtained following the MethylDetect BRCA2 kit protocol, using the LightCycler® 480 High Resolution Melting Master. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.

Additional information

C

A, B